Collagen from bovine achilles tendon 膠原蛋白I型說明書
Product Number: C8060
CAS Number : 9007-34-5
Storage Temperature: 2-8°C
Product Description
This product is suitable for use as a substrate for collagenase. It is prepared by modification of the method of Einbinder
and Schubert and is not suitable for use in coating glassware.
This collagen is Bornstein and Traub Type I. The Type I classification of collagen is a component of skin, bone, tendon,
and other fibrous connective tissues. Type I collagen differs from other collagens by their low lysine hydroxylation
and low carbohydrate composition.
Collagen breaks down metabolically in the body to release N-opeptide, which is the N-terminus of collagen. There
is also C-opeptide, which is presumably the C-terminus. N-opeptide is released in urine, and its detection in
diagnostic tests is used to screen for osteoporosis.
Although different types of collagen exist, they are all composed of molecules containing three polypeptide chains
arranged in a triple helical conformation. Slight differences in the primary structure (amino acid sequence) establish
differences between the types. The amino acid sequence of the primary structure is mainly a repeating motif with
glycine in every third position and proline or 4-hydroxyproline frequently preceeding the glycine residue.
Preparation Instructions
This product is an insoluble collagen preparation. It is insoluble in water, aqueous buffers, dilute acid, and organic
solvents. For use as a substrate in collagenase assays, this collagen can be prepared as a suspension in 50 mM TES
buffer, pH 7.4 with 0.36 mM calcium chloride. but not a clear solution.
Storage/Stability
It is recommended to store this product at 2–8 °C. If stored as recommended, it will have a shelf-life of up to 8 years.
Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or other uses.
Solarbio
Beijing Solarbio Science & Technology Co., Ltd
Http://www.solarbio-hd。。ｃｎ
產品數量:C8060
CAS編號:9007-34-5
存儲溫度:2 - 8°C
產品描述
本產品適用于使用作為膠原酶的底物。它是由Einbinder的修改方法和舒伯特,并不適用于鍍膜玻璃器皿。這個膠原蛋白是波恩和特勞布類型一、I型膠原蛋白是皮膚的一個組成部分,分類骨骼,肌腱,
和其他纖維結締組織。I型膠原蛋白不同于其他膠原蛋白低賴氨酸羥基化和低碳水化合物組成。
膠原蛋白在體內分解代謝釋放N-opeptide,膠原蛋白分子的n端。有也是C-opeptide,想必C-terminus。尿液N-opeptide釋放,其檢測診斷測試篩選用于骨質疏松癥。
盡管不同類型的膠原蛋白存在,他們都包含三個多肽鏈組成的分子
安排在一個三重螺旋構象。輕微的差異(氨基酸序列)建立的主要結構
類型之間的差異。的氨基酸序列一級結構主要是一個重復的主題
在每個第三位置和脯氨酸或甘氨酸4-hydroxyproline經常在甘氨酸殘基前夕。
本產品是一種不溶性膠原蛋白制備。它不溶于水,水溶液緩沖區,稀酸和有機的
溶劑。作為基質的膠原酶化驗,這種膠原蛋白可以作為懸掛在50 mM的te做好準備
緩沖區,pH值7.4與0.36毫米氯化鈣。但不是一個明確的解決方案。
存儲/穩定
建議儲存該產品在2 - 8°C。如果存儲為建議,它將有8年的保質期。
預防措施和免責聲明
僅供實驗室使用。不是藥物,家庭或其他用途。
